ViroVision™ Rev-CEM-GFP HIV Reporter Cells (CUBRC0031)

Product Name: ViroVision™ Rev-CEM-GFP HIV Reporter Cells
Catalog No: CUBRC0031

Infection of Rev-CEM-GFP by HIV results in GFP expression in 2-5 days. Rev-CEM-GFP is permissive to a multi-subtype range of X4 utilizing primary isolates and infectious molecular clones. Rev-CEM-GFP has no background GFP expression in the absence of HIV infection and is resistant to non-viral stimulations by mitogens, cytokines, or other cellular activators.

Quantity: One vial of 5 x 10⁶ cells.
Progenitor: Human T4-lymphoblastoid cell line, CEM-SS.
Phenotype: Expresses CD4 and CXCR4.
Applications: TCID50 assays, routine measurements of viral infectivity, anti-HIV drug screening, anti-HIV neutralizing antibody screening and quantification, HIV cell-cell transmission, HIV+ cell-derived exosomes.
Freezing Media: ViroVision™ Freezing Media: 93% FBS/RPMI with 7% DMSO.
Propagation Media: ViroVision™ Growth Media C: RPMI, 10% FBS.
Growth: Split 1:2 every 1-2 days, or 1:10-1:20 every 2-3 days.
Morphology: Round, Clusters
Detection: Fluorescent microscopy or flow cytometry.
Shipping Conditions: Cells are shipped frozen. If cells are not frozen upon arrival, contact Cube Biosystems immediately.
Storage Conditions: Store cells in liquid nitrogen until ready to propagate. Store Infection Enhancment Media at -20°C until ready to use. Once thawed, Infection media is good for 3 months and should be stored at 4°C.
Warranty: Cube Biosystems warrants that cells shall be viable upon shipment from Cube Biosystems for a period of thirty days, provided they have been properly stored and handled during this period.
Safety: BSL 1: Appropriate safety procedures should always be used with this material. Prior to thawing cells, under sterile conditions: Unscrew cap 1/4 to 1/2 turn to allow N2 to escape. Re-secure cap.
Quality: Negative for mycoplasma, bacteria and fungi.
Disclaimer: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
License: PDF:        Web:  Click Here
Directions for Use: PDF:        Web:  Click Here
Data Sheet: PDF:  
Storage Instructions: Store cells in vapor phase of liquid nitrogen until you are ready to thaw and propagate.
Wu, Y., Beddall, M. H., and Marsh, J. W. (2007). Rev-dependent indicator T cell line. Current HIV Research 5, 395-403.

Write a review

Please login or register to review

Related Products

Cat No.: CUBRC0031
Size Price
One vial of 5 x 10⁶ cells. $899.00
Shipping info


Antibodies, Proteins, ELISA Kits: 5 to 10 Business Days, Ambient (unless otherwise required*)
*Based on stability testing and to save packing waste, most antibodies will be shipped at ambient temperature.

Cells: 2 to 3 Days, Dry Ice.


Ambient:  $25

Blue Ice/Ice Packs:  $45 (As Required or Upon request)

Dry Ice: $55



Order Info