Product Name: | ViroVision™ Rev-A3R5-GFP/Luc HIV Reporter Cells |
Catalog No: | CUBRC0012 |
Description: | Infection of Rev-A3R5-GFP/Luc by HIV results in GFP/Luc expression in 2-5 days. Rev-A3R5-GFP/Luc is permissive to a multi-subtype range of X4 and R5 utilizing primary isolates and infectious molecular clones. Rev-A3R5-GFP/Luc has no background GFP expression in the absence of HIV infection and is resistant to non-viral stimulations by mitogens, cytokines, or other cellular activators. |
Quantity: | One vial of 5 x 10⁶ cells. |
Progenitor: | Human T4-lymphoblastoid cell line, A3.01 |
Phenotype: | Expresses CD4, CXCR4 and α4β7 and constitutively expresses CCR5. |
Applications: | TCID50 assays, routine measurements of viral infectivity, anti-HIV drug screening, anti-HIV neutralizing antibody screening and quantification, HIV cell-cell transmission, HIV+ cell-derived exosomes. |
Freezing Media: | ViroVision™ Freezing Media: 93% FBS/RPMI with 7% DMSO. |
Propagation Media: | ViroVision™ Growth Media A: RPMI-1640 containing 10-15% FBS, 1% L-Glut, 1% Pen/Strep, and 1 mg/mL Geneticin (G418) |
Growth: | Split 1:5, twice per week. Cells ready for infection after 2nd passage. |
Morphology: | Round |
Detection: | Fluorescent microscopy or flow cytometetry (GFP). Luminometer and luciferase detection system (Luc). |
Shipping Conditions: | Cells are shipped frozen. If cells are not frozen upon arrival, contact Cube Biosystems immediately. |
Storage Conditions: | Store cells in liquid nitrogen until ready to propagate. Store Infection Enhancment Media at -20°C until ready to use. Once thawed, Infection media is good for 3 months and should be stored at 4°C. |
Warranty: | Cube Biosystems warrants that cells shall be viable upon shipment from Cube Biosystems for a period of thirty days, provided they have been properly stored and handled during this period. |
Safety: | BSL 1: Appropriate safety procedures should always be used with this material. Prior to thawing cells, under sterile conditions: Unscrew cap 1/4 to 1/2 turn to allow N2 to escape. Re-secure cap. |
Quality: | Negative for mycoplasma, bacteria and fungi. |
Disclaimer: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. |
License: | PDF: Web: Click Here |
Directions for Use: | PDF: Web: Click Here |
Data Sheet: | PDF: |
Storage Instructions: | Store cells in vapor phase of liquid nitrogen until you are ready to thaw and propagate. |