Overview
| Product Name: | Human ALCAM ELISA Kit |
| Catalog No: | CUBEK01485 |
| Description: | For quantitative detection of human ALCAM in cell culture supernatants, serum and plasma (heparin , EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ALCAM. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: W28 - A526 |
| Pinciple: | This human TGF-beta 3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 3 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTGF-beta 3 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human TGF-beta 3 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | ALCAM |
| Background: | CD166 antigen is a 100 - 105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1 - q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC - 1/DM - GRASP/BEN in the chicken, and KG - CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker. |
| Synonym: | ALCAM protein ; CD166 antigen ; Protein MEMD ; Q13740 |
| NCBI Gene ID: | 214 |
| UniProt ID: | Q13740 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 3.07 | 6.42 | 13.26 | 3.58 | 7.04 | 14.98 |
| Standard deviation: | 0.150 | 0.327 | 0.756 | 0.218 | 0.444 | 0.959 |
| CV (%): | 4.9 | 5.1 | 5.7 | 6.1 | 6.3 | 6.4 |
Typical Data
| Concentration (pg/ml): | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D.: | 0.033 | 0.109 | 0.137 | 0.215 | 0.385 | 0.688 | 1.138 | 2.063 |
Images
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Human ALCAM ELISA Kit standard curve |
Review