Overview
| Product Name: | Human Adiponectin ELISA Kit |
| Catalog No: | CUBEK01222 |
| Description: | For quantitative detection of human Adiponectin in cell culture supernatants, serum, plasma (heparin, EDTA), tissue homogenates and urine. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human total Adiponectin. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: E19 - N244 |
| Pinciple: | This human TSP2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TSP2 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTSP2 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human TSP2 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 60 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | Adiponectin |
| Background: | Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor - dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19 - 244) amino acids with the molecular mass of 36KDa after glycosylation. |
| Synonym: | 30 kDa adipocyte complement related protein; 30 kDa adipocyte complement - related protein; ACDC; ACRP30; ADIPO_HUMAN; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipocyte complement - related 30 kDa protein; Adiponectin; Adipoq; Adipose most abundant gene transcript 1; Adipose most abundant gene transcript 1 protein; Adipose specific collagen like factor; ADIPQTL1; ADPN; apM - 1; APM1; C1q and collagen domain - containing protein; GBP28; Gelatin binding protein; Gelatin binding protein 28; Gelatin - binding protein |
| NCBI Gene ID: | 9370 |
| UniProt ID: | A8K660 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 13.8 | 37.2 | 67.3 | 13.1 | 36.8 | 65.7 |
| Standard deviation: | 0.39 | 1.34 | 3.03 | 0.77 | 2.50 | 4.66 |
| CV (%): | 2.8 | 3.6 | 4.5 | 5.9 | 6.8 | 7.1 |
Typical Data
| Concentration (ng/ml): | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D.: | 0.003 | 0.074 | 0.149 | 0.369 | 0.509 | 0.862 | 1.510 | 2.059 |
Images
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Human Adiponectin ELISA Kit standard curve |
Review