Overview
| Product Name: | Human ADAMTS4 ELISA Kit |
| Catalog No: | CUBEK01697 |
| Description: | For quantitative detection of human ADAMTS4 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ADAMTS4. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: CHO; Immunogen sequence: F213 - C685 |
| Pinciple: | This human LOXL2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LOXL2 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forLOXL2 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human LOXL2 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 20 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | ADAMTS4 |
| Background: | ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc - dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C - terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain - specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. |
| Synonym: | A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4; A disintegrin like and metalloprotease with thrombospondin type 1 motif 4; ADAM metallopeptidase with thrombospondin type 1 motif 4; ADAM TS 4; ADAM TS4; ADAM - TS 4; ADAM - TS4; ADAMTS 2; ADAMTS 4; ADAMTS 4; ADAMTS - 4; ADAMTS2; ADAMTS4; ADAMTS4 protein; ADMP 1; ADMP - 1; ADMP1; Aggrecanase 1; Aggrecanase 1; Aggrecanase - 1; Aggrecanase1; ATS4_HUMAN; KIAA0688; KIAA0688 |
| NCBI Gene ID: | 9507 |
| UniProt ID: | O75173 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 5.9 | 12 | 21 | 6.5 | 14 | 23 |
| Standard deviation: | 0.248 | 0.612 | 1.2 | 0.377 | 0.882 | 1.66 |
| CV (%): | 4.2 | 5.1 | 5.7 | 5.8 | 6.3 | 7.2 |
Typical Data
| Concentration (pg/ml): | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D.: | 0.025 | 0.106 | 0.182 | 0.301 | 0.535 | 1.005 | 1.655 | 2.265 |
Review