Overview
| Product Name: | Human ADAMTS13 ELISA Kit |
| Catalog No: | CUBEK01427 |
| Description: | For quantitative detection of human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ADAMTS13. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: CHO; Immunogen sequence: Q34 - W688 |
| Pinciple: | This human ADAM12 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM12 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forADAM12 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human ADAM12 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 20 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | ADAMTS13 |
| Background: | ADAMTS13 is a zinc - containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal - containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor - cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity. |
| Synonym: | A disintegrin and metalloproteinase with thrombospondin motifs 13; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13; A disintegrin like and metalloprotease with thrombospondin type 1 motif 13; A disintegrin - like and metalloprotease (Reprolysin type) with thrombospondin type 1 motif 13; ADAM metallopeptidase with thrombospondin type 1 motif 13; ADAM TS; ADAM - TS 13; ADAM - TS13; ADAMTS 13; ADAMTS - 13; ADAMTS13; ADAMTS13 protein; ATS13_HUMAN; C9orf8; EC 3.4.24. - ; TTP; Von Willebrand factor cleaving protease; Von Willebrand factor cleaving protease; von Willebrand factor - cleaving protease; vWF cleaving protease; vWF CP; vWF - cleaving protease; vWF - CP; vWFCP |
| NCBI Gene ID: | 11093 |
| UniProt ID: | Q76LX8 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 5.62 | 10.1 | 25.5 | 6.23 | 12.1 | 26.4 |
| Standard deviation: | 0.259 | 0.444 | 1.07 | 0.467 | 0.835 | 1.95 |
| CV (%): | 4.6 | 4.4 | 4.2 | 7.5 | 6.9 | 7.4 |
Typical Data
| Concentration (ng/ml): | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D.: | 0.051 | 0.101 | 0.134\0.243 | 0.439 | 0.829 | 1.482 | 2.472 |
Review