Overview
| Product Name: | Human ADAMTS1 ELISA Kit |
| Catalog No: | CUBEK01669 |
| Description: | For quantitative detection of human ADAMTS1 in cell culture supernatants, serum and plasma (heparin). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ADAMTS1. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: F253 - A734 |
| Pinciple: | This human JAM-A ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for JAM-A has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forJAM-A is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human JAM-A within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | ADAMTS1 |
| Background: | ADAMTS1 (A Disintegrin - Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. |
| Synonym: | A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin like and metalloprotease with thrombospondin type 1 motif 1; A disintegrin - like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1; ADAM metallopeptidase with thrombospondin type 1 motif 1; ADAM TS 1; ADAM TS1; ADAM - TS 1; ADAM - TS1; ADAMTS 1; ADAMTS; ADAMTS - 1; Adamts1; ATS1_HUMAN; C3 C5; KIAA1346; METH 1; METH - 1; METH1 |
| NCBI Gene ID: | 9510 |
| UniProt ID: | Q9UHI8 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 1208 | 2741 | 4137 | 1850 | 3035 | 4726 |
| Standard deviation: | 42.28 | 117.9 | 202.7 | 98.05 | 176.03 | 302.5 |
| CV (%): | 3.5 | 4.3 | 4.9 | 5.3 | 5.8 | 6.4 |
Typical Data
| Concentration (pg/ml): | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D.: | 0.026 | 0.096 | 0.160 | 0.278 | 0.513 | 0.920 | 1.599 | 2.476 |
Review