Overview
| Product Name: | Human ADAM8 ELISA Kit |
| Catalog No: | CUBEK01229 |
| Description: | For quantitative detection of human ADAM8 in cell culture supernatants and serum. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ADAM8. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: M1 - P497 |
| Pinciple: | This mouse AXL ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for AXL has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forAXL is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse AXL within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
|
Material Required But Not Provided
|
Background
| Gene Name: | ADAM8 |
| Background: | A Disintegrin and metalloproteinase domain - containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane - bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration. |
| Synonym: | A Disintegrin And Metalloproteinase domain 8; A Disintegrin And Metalloproteinase domain 8; ADAM 8; ADAM 8 precursor; ADAM 8 precursor; ADAM metallopeptidase domain 8; Adam8; ADAM8 protein; ADAM8_HUMAN; CD 156; CD156; CD156a; CD156a antigen; CD156a antigen; Cell surface antigen MS2; Cell surface antigen MS2; Disintegrin and metalloproteinase domain - containing protein 8; Human leukocyte differentiation antigen; Human leukocyte differentiation antigen; Macrophage cysteine rich glycoprotein; Macrophage cysteine rich glycoprotein; MGC134985; MS 2; MS2 |
| NCBI Gene ID: | 101 |
| UniProt ID: | P78325 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 0.22 | 1.47 | 2.34 | 0.27 | 1.56 | 2.58 |
| Standard deviation: | 0.013 | 0.090 | 0.185 | 0.018 | 0.115 | 0.214 |
| CV (%): | 5.7 | 6.1 | 7.9 | 6.8 | 7.4 | 8.3 |
Typical Data
| Concentration (pg/ml): | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D.: | 0.006 | 0.060 | 0.129 | 0.256 | 0.442 | 0.829 | 1.384 | 1.979 |
Review