Overview
| Product Name: | Human ACE ELISA Kit |
| Catalog No: | CUBEK01193 |
| Description: | For quantitative detection of human ACE in cell culture supernatants, serum, plasma (heparin) and saliva. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ACE. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: L30 - L1261 |
| Pinciple: | This human CT-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CT-1 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forCT-1 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human CT-1 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 5 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | ACE |
| Background: | Angiotensin - converting enzyme (ACE) is a zinc - containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C - terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732 - residue preprotein including a 31 - residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin - converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30 - 1261 amino acids with the molecular mass of 120KDa. |
| Synonym: | ACE 1 ; ACE T ; Angiotensin converting enzyme ; Angiotensin Converting Enzyme 1 ; Angiotensinconverting enzyme ; Angiotensin - converting enzyme ; CD143 antigen ; DCP 1 ; DCP, DCP1 ; Dipeptidyl carboxypeptidase 1 ; Dipeptidyl carboxypeptidase I ; Kininase II ; MVCD3 ; P12821 ; Peptidyl dipeptidase A |
| NCBI Gene ID: | 1636 |
| UniProt ID: | B4DKH4 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 0.85 | 2.73 | 6.87 | 0.97 | 3.42 | 6.33 |
| Standard deviation: | 0.036 | 0.112 | 0.261 | 0.671 | 0.246 | 0.335 |
| CV (%): | 4.2 | 4.1 | 3.8 | 6.9 | 7.2 | 5.3 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.051 | 0.119 | 0.204 | 0.336 | 0.611 | 0.929 | 1.447 | 2.154 |
Images
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Human ACE ELISA Kit standard curve |
Review