Overview
| Product Name: | Rat TrkA ELISA Kit |
| Catalog No: | CUBEK01372 |
| Description: | For quantitative detection of rat TrkA in cell culture supernatants, cell lysates and tissue homogenates. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Rat |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant rat TrkA. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: A33 - P418 |
| Pinciple: | This human NGAL ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGAL has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forNGAL is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human NGAL within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | TrkA |
| Background: | Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild - type receptor.2 TrkA is part of a sub - family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF (for Brain - Derived Neurotrophic Factor), NT - 3 (for Neurotrophin - 3) and NT - 4 (for Neurotrophin - 4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT - 4, and NT - 3. Further, TrkC binds and is activated by NT - 3.3 TrkA receptor was found in keratoconus - affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part (A33 - P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa. |
| Synonym: | High affinity nerve growth factor receptor; MTC; Neurotrophic tyrosine kinase receptor type 1; NTRK1; NTRK1_HUMAN; Oncogene TRK; p140 - TrkA; TRK; Trk - A; TRK1; TRK1 - transforming tyrosine kinase protein |
| NCBI Gene ID: | 59109 |
| UniProt ID: | P35739 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 595 | 3622 | 6653 | 614 | 3125 | 7234 |
| Standard deviation: | 32.13 | 166.6 | 452.4 | 38.68 | 162.5 | 542.6 |
| CV (%): | 5.4 | 4.6 | 6.8 | 6.3 | 5.2 | 7.5 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.006 | 0.146 | 0.252 | 0.509 | 0.948 | 1.353 | 1.903 | 2.378 |
Images
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Rat TrkA ELISA Kit standard curve |
Review