Overview
| Product Name: | Rat TNFSF14/LIGHT ELISA Kit |
| Catalog No: | CUBEK01824 |
| Description: | For quantitative detection of rat TNFSF14 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Rat |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant rat TNFSF14. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: I82 - I239 |
| Pinciple: | This rat TNFSF14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF14 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: I82-I239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TNFSF14 amount of sample captured in plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | TNFSF14 |
| Background: | Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. |
| Synonym: | CD258; Delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator A; Herpesvirus entry mediator ligand; HVEM L; HVEM - L; HVE ml; Ligand for herpesvirus entry mediator; LTg; TNF14_HUMAN; Tnfsf14; Tumor necrosis factor (ligand) superfamily member 14; Tumor necrosis factor ligand superfamily member 14; Tumor necrosis factor receptor like 2; Tumor necrosis factor superfamily member LIGHT |
| NCBI Gene ID: | 301133 |
| UniProt ID: | M0RCN2 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 151 | 520 | 1617 | 172 | 499 | 2453 |
| Standard deviation: | 10.26 | 33.28 | 103.48 | 8.77 | 24.45 | 166.80 |
| CV (%): | 6.8 | 6.4 | 6.4 | 5.1 | 4.9 | 6.8 |
Typical Data
| Concentration (pg/ml): | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D.: | 0.011 | 0.090 | 0.169 | 0.318 | 0.581 | 1.077 | 1.665 | 2.359 |
Review