Overview
| Product Name: | Rat TNF alpha ELISA Kit |
| Catalog No: | CUBEK01174 |
| Description: | For quantitative detection of rat TNF alpha in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Rat |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant rat TNF alpha. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: E.coli; Immunogen sequence: L80 - L235 |
| Pinciple: | This mouse TNFR2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFR2 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTNFR2 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse TNFR2 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 1 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | TNF alpha |
| Background: | Tumor necrosis factor - alpha (TNF - alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF - beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF - alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte - macrophage colony - stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3 - kilodalton protein from HL - 60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF - alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa. |
| Synonym: | APC1 protein; Cachectin; DIF; soluble form; Tnf; TNF a; TNF alpha; TNF macrophage derived; TNF monocyte derived; TNF superfamily member 2; TNF - a; TNF - alpha; TNFA_HUMAN; TNFSF2; Tumor necrosis factor; Tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2 |
| NCBI Gene ID: | 24835 |
| UniProt ID: | P16599 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 90 | 245 | 612 | 103 | 261 | 684 |
| Standard deviation: | 4.77 | 7.6 | 20.81 | 6.9 | 15.4 | 44.5 |
| CV (%): | 5.3 | 3.1 | 3.4 | 6.7 | 5.9 | 6.5 |
Typical Data
| Concentration (pg/ml): | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D.: | 0.010 | 0.061 | 0.117 | 0.225 | 0.443 | 0.833 | 1.645 | 2.481 |
Images
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Rat TNF alpha ELISA Kit standard curve |
Review