Overview
| Product Name: | Rat TGF-beta 3 ELISA Kit |
| Catalog No: | CUBEK01494 |
| Description: | For quantitative detection of activated rat TGF - beta 3 in cell culture supernatants, serum, plasma (EDTA) and urine. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Rat |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant rat TGF-beta 3. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: sf21; Immunogen sequence: A301 - S412 |
| Pinciple: | This human Elafin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Elafin has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forElafin is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human Elafin within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | TGF-beta 3 |
| Background: | Transforming growth factor beta 3 (TGF - beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF - beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF - beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF - beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF - beta 3 activated Lef1 in the absence of beta - catenin (CTNNB1) via nuclear phospho - Smad2 and Smad4. |
| Synonym: | ARVD; ARVD1; FLJ16571; MGC105479; MGC118722; prepro - transforming growth factor beta - 3; RNHF; TGF beta 3; TGF beta3; TGF - beta - 3; TGFB 3; TGFB3; TGFB3_HUMAN; transforming growth factor beta 3; Transforming growth factor beta - 3 |
| NCBI Gene ID: | 25717 |
| UniProt ID: | Q07258 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 240 | 528 | 1024 | 256 | 545 | 1118 |
| Standard deviation: | 9.84 | 25.9 | 56.32 | 13.3 | 32.7 | 75 |
| CV (%): | 4.1 | 4.9 | 5.5 | 5.2 | 6 | 6.7 |
Typical Data
| Concentration (pg/ml): | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D.: | 0.018 | 0.126 | 0.223 | 0.430 | 0.812 | 1.379 | 2.135 | 2.505 |
Images
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Rat TGF-beta 3 ELISA Kit standard curve |
Review