Overview
| Product Name: | Rat TGF beta 1 ELISA Kit |
| Catalog No: | CUBEK01164 |
| Description: | For quantitative detection of activated rat TGF beta 1 in cell culture supernatants, serum, plasma (EDTA) and urine. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Rat |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant rat TGF beta 1. There is cross-reactivates with TGFbeta2, TGFbeta3, TGFbeta5< 1%. |
| Standard Source: | Expression system for standard: CHO; Immunogen sequence: A279 - S390 |
| Pinciple: | This mouse TIMP-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-1 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTIMP-1 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse TIMP-1 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 1 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | TGF beta 1 |
| Background: | Transforming growth factor - beta1 (TGF - beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF - beta and essentially all of them have specific receptors for this peptide. TGF - beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF - beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF - beta1 may also regulate blood pressure via stimulation of endothelin - 1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency - associated peptide (beta1 - LAP) in either the presence or the absence of additional latent TGF - beta1 - binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa. |
| Synonym: | Camurati - Engelmann disease; CED; DPD1; LAP; Latency - associated peptide; TGF beta 1 protein; TGF - beta - 1; TGFB; tgfb1; TGFB1_HUMAN; TGFbeta; Transforming growth factor beta 1 |
| NCBI Gene ID: | 59086 |
| UniProt ID: | P17246 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 122 | 253 | 506 | 140 | 256 | 613 |
| Standard deviation: | 4.15 | 11.4 | 25.81 | 6.72 | 14.08 | 37.39 |
| CV (%): | 3.4 | 4.5 | 5.1 | 4.8 | 5.5 | 6.1 |
Typical Data
| Concentration (pg/ml): | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D.: | 0.039 | 0.108 | 0.186 | 0.321 | 0.599 | 1.017 | 1.586 | 2.200 |
Images
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Rat TGF beta 1 ELISA Kit standard curve |
Review