Overview
| Product Name: | Human APP ELISA Kit |
| Catalog No: | CUBEK01232 |
| Description: | For quantitative detection of human APP in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human APP. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: L18 - L688 |
| Pinciple: | This human BAFF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BAFF has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forBAFF is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human BAFF within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | APP |
| Background: | Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (Abeta), a 39 - to 42 - amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha - , beta - , and gamma - secretases. Alpha - secretase generates soluble amyloid protein, while beta - and gamma - secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive. |
| Synonym: | A4 amyloid protein; A4; A4_HUMAN; AAA; ABETA; ABPP; AD 1; AD1; AICD - 50; AICD - 57; AICD - 59; AID (50); AID (57); AID (59); Alzheimer disease 1; Alzheimer disease amyloid protein; Alzheimer's disease amyloid protein; Amyloid beta (A4) precursor protein; Amyloid beta A4 protein; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta A4 protein precursor isoform c; Amyloid beta A4 protein precursor isoform a; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta A4 protein precursor isoform c; Amyloid beta protein; Amyloid beta - peptide; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; Amyloid of aging and alzheimer disease; APP; APP I; APPI; Beta amyloid peptide; Beta - APP40; Beta - APP42; C31; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Gamma - CTF (50); Gamma - CTF (57); Gamma - CTF (59); Human mRNA for amyloid A4 precursor of Alzheimer's disease; peptidase nexin II; PN 2; PN II; PN - II; PN2; PreA4; Protease nexin II; Protease nexin - II; S - APP - alpha; S - APP - beta |
| NCBI Gene ID: | 351 |
| UniProt ID: | P05067 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 3.37 | 6.58 | 13.29 | 3.42 | 6.68 | 14.21 |
| Standard deviation: | 0.162 | 0.336 | 0.704 | 0.198 | 0.414 | 0.895 |
| CV (%): | 4.8 | 5.1 | 5.3 | 5.8 | 6.2 | 6.3 |
Typical Data
| Concentration (pg/ml): | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D.: | 0.130 | 0.181 | 0.221 | 0.323 | 0.506 | 0.788 | 1.248 | 1.854 |
Images
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Human APP ELISA Kit standard curve |
Review