Overview
| Product Name: | Human APOE ELISA Kit |
| Catalog No: | CUBEK01752 |
| Description: | For quantitative detection of human APOE in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, milk and urine. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural human APOE. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: E.coli; Immunogen sequence: K19 - H317 |
| Pinciple: | This human HE4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HE4 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forHE4 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human HE4 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 50 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | APOE |
| Background: | Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate - density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride - rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform - dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease. |
| Synonym: | AD2; Alzheimer disease 2; Apo E; Apo - E; ApoE; APOEA; Apolipoprotein E; Apolipoprotein - E; ApolipoproteinE; Apoprotein; LDLCQ5; L pg |
| NCBI Gene ID: | 348 |
| UniProt ID: | P02649 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 13.6 | 52 | 124 | 18.9 | 78 | 148 |
| Standard deviation: | 0.68 | 2.91 | 7.56 | 0.98 | 4.9 | 10.66 |
| CV (%): | 5.0 | 5.6 | 6.1 | 5.2 | 6.4 | 7.2 |
Typical Data
| Concentration (ng/ml): | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D.: | 0.010 | 0.073 | 0.138 | 0.243 | 0.484 | 0.907 | 1.475 | 2.204 |
Review