Overview
| Product Name: | Human Angiopoietin-1 ELISA Kit |
| Catalog No: | CUBEK01195 |
| Description: | For quantitative detection of human Angiopoietin - 1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human Angiopoietin-1. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: H16 - F498 |
| Pinciple: | This mouse CCL1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL1 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forCCL1 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse CCL1 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | Angiopoietin-1 |
| Background: | Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell - specific tyrosine - protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. |
| Synonym: | 1110046O21Rik; AGP 1; AGP1; AGPT; ANG 1; ANG1; Angiopoietin1; ANGPT 1; ANGPT1; KIAA0003 |
| NCBI Gene ID: | 284 |
| UniProt ID: | Q15389 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 0.97 | 3.05 | 6.32 | 1.12 | 3.37 | 6.58 |
| Standard deviation: | 0.042 | 0.116 | 0.259 | 0.073 | 0.179 | 0.368 |
| CV (%): | 4.3 | 3.8 | 4.1 | 6.5 | 5.3 | 5.6 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.092 | 0.118 | 0.147 | 0.233 | 0.375 | 0.684 | 1.227 | 2.137 |
Images
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Human Angiopoietin-1 ELISA Kit standard curve |
Review