Overview
| Product Name: | Human Amphiregulin (AR) ELISA Kit |
| Catalog No: | CUBEK01004 |
| Description: | For quantitative detection of human Amphiregulin in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human Amphiregulin. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: E.coli; Immunogen sequence: S101 - K198 |
| Pinciple: | This human Amphiregulin (AR) ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Amphiregulin has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forAmphiregulin is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human Amphiregulin (AR) within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
|
Material Required But Not Provided
|
Background
| Gene Name: | Amphiregulin (AR) |
| Background: | Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF - alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis - like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty - induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes. |
| Synonym: | A REG; AR; AREG; Colorectum cell derived growth factor; CRDGF; MGC13647; OTTHUMP00000219184; Schwannoma derived growth factor; SDGF |
| NCBI Gene ID: | 374 |
| UniProt ID: | P15514 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 115 | 351 | 633 | 104 | 339 | 620 |
| Standard deviation: | 6.67 | 15.1 | 29.75 | 8.94 | 24.75 | 32.24 |
| CV (%): | 5.8 | 4.3 | 4.7 | 8.6 | 7.3 | 5.2 |
Typical Data
| Concentration (pg/ml): | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D.: | 0.033 | 0.104 | 0.173 | 0.305 | 0.576 | 1.178 | 1.834 | 2.353 |
Images
 |
Human Amphiregulin(AR) ELISA Kit standard curve |
Review