Overview
| Product Name: | Mouse PECAM-1/CD31 ELISA Kit |
| Catalog No: | CUBEK01392 |
| Description: | For quantitative detection of mouse PECAM - 1 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Mouse |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant mouse PECAM-1. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: E18 - K590 |
| Pinciple: | This human Endostatin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Endostatin has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forEndostatin is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human Endostatin within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | PECAM-1 |
| Background: | PECAM - 1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule - 1 (PECAM - 1) is a 130 - kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T - cell subsets.2 Platelet endothelial cell adhesion molecule - 1 (PECAM - 1/CD31) is a member of the immunoglobulin (Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs (ITIMs) in its ligand binding polypeptide.3 PECAM - 1 has recently been shown to contain functional immunoreceptor tyrosine - based inhibitory motifs (ITIMs) within its cytoplasmic domain, and co - ligation of PECAM - 1 with the T - cell antigen receptor (TCR) results in tyrosine phosphorylation of PECAM - 1, recruitment of Src homology 2 domain - containing protein tyrosine phosphatase - 2 (SHP - 2), and attenuation of TCR - mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 563 amino acids (E28 - K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91 - 100KDa. |
| Synonym: | CD31; CD31 antigen; EndoCAM; GPIIA; HEC7; Leukocyte surface protein; PECA1; PECA1_HUMAN; PECAM 1; PECAM - 1; Pecam1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule |
| NCBI Gene ID: | 18613 |
| UniProt ID: | Q08481 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 1425 | 2256 | 4926 | 1614 | 2648 | 5830 |
| Standard deviation: | 89.8 | 169.2 | 280.8 | 111.4 | 214.5 | 378.95 |
| CV (%): | 6.3 | 7.5 | 5.7 | 6.9 | 8.1 | 6.5 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.032 | 0.149 | 0.245 | 0.413 | 0.751 | 1.246 | 1.952 | 2.170 |
Images
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Mouse PECAM-1/CD31 ELISA Kit standard curve |
Review