Overview
Properties
Sensitivity: | < 10 pg/ml |
Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
Gene Name: | Cystatin B |
Background: | Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell. |
Synonym: | CHROW21; CPI B; CPI - B; CST6; CSTB; Cystatin B; Cystatin - B; CYTB; CYTB_HUMAN; EPM1; EPM1A; Liver thiol proteinase inhibitor; PME; Stefin - B; STFB; ULD |
NCBI Gene ID: | 1476 |
UniProt ID: | P04080 |
Intra/Inter Assay Precision
Intra | Inter | |||||
Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
n: | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml): | 421 | 1169 | 2311 | 428 | 1207 | 2365 |
Standard deviation: | 26.1 | 67.8 | 140.97 | 30.82 | 82.08 | 165.6 |
CV (%): | 6.2 | 5.8 | 6.1 | 7.2 | 6.8 | 7 |
Typical Data
Concentration (pg/ml): | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D.: | 0.055 | 0.093 | 0.153 | 0.248 | 0.44 | 0.821 | 1.349 | 2.075 |
Images
Human Cystatin B ELISA Kit standard curve |
Review