Overview
Properties
Sensitivity: | < 20 pg/ml |
Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
Gene Name: | A2M |
Background: | Alpha - 2 - macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha - 2 - macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A - beta, the major component of beta - amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. |
Synonym: | C3 and PZP - like alpha - 2 - macroglobulin domain - containing protein 5; A2m; A2MD; A2MG_HUMAN; Alpha - 2 - M; Alpha - 2 - macroglobulin; C3 and PZP - like alpha - 2 - macroglobulin domain - containing protein 5; CPAMD5; FWP007; Pregnancy zone protein; S863 - 7 |
NCBI Gene ID: | 2 |
UniProt ID: | P01023 |
Intra/Inter Assay Precision
Intra | Inter | |||||
Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
n: | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (ng/ml): | 6.18 | 12.04 | 25.21 | 6.72 | 13.68 | 26.81 |
Standard deviation: | 0.377 | 0.771 | 0.174 | 0.457 | 0.985 | 2.12 |
CV (%): | 6.1 | 6.4 | 6.9 | 6.8 | 7.2 | 7.9 |
Typical Data
Concentration (pg/ml): | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D.: | 0.075 | 0.129 | 0.203 | 0.332 | 0.575 | 1.108 | 1.477 | 2.135 |
Images
Human A2M/alpha2-Macroglobulin ELISA Kit standard curve |
Review