Overview
| Product Name: | Rat VEGF ELISA Kit |
| Catalog No: | CUBEK01186 |
| Description: | For quantitative detection of rat VEGF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Rat |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant rat VEGF. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: A27 - R190 |
| Pinciple: | This human CCL21 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL21 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forCCL21 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human CCL21 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 1 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
|
Material Required But Not Provided
|
Background
| Gene Name: | VEGF |
| Background: | Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk - 1 and Flt - 1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer. |
| Synonym: | MGC70609; MVCD1; Vascular endothelial growth factor; Vascular endothelial growth factor A; Vascular permeability factor; VEGF A; VEGFA; VPF |
| NCBI Gene ID: | 83785 |
| UniProt ID: | B5DEK7 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 120 | 305 | 621 | 236 | 289 | 632 |
| Standard deviation: | 4.44 | 15.86 | 29.81 | 10.62 | 18.21 | 34.76 |
| CV (%): | 3.7 | 5.2 | 4.8 | 4.5 | 6.3 | 5.5 |
Typical Data
| Concentration (pg/ml): | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D.: | 0.023 | 0.075 | 0.119 | 0.234 | 0.401 | 0.804 | 1.433 | 1.971 |
Images
 |
Rat VEGF ELISA Kit standard curve |
Review