Overview
| Product Name: | Rat TIMP-2 ELISA Kit |
| Catalog No: | CUBEK01640 |
| Description: | For quantitative detection of rat TIMP - 2 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Rat |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant rat TIMP-2. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: C27 - P220 |
| Pinciple: | This mouse CD70 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD70 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forCD70 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse CD70 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | TIMP-2 |
| Background: | TIMP - 2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP - 2 is 83 kilobase pairs (kb) long with exon - intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases - 2 is localized on human chromosome arm 17q25. TIMP - 2 abrogates angiogenic factor - induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP - 2 with the molecular mass of 22Kda and 194 amino acid. |
| Synonym: | CSC 21K; CSC21K; D11Bwg1104e; DDC8; Metalloproteinase inhibitor 2; Metalloproteinase inhibitor 2 precursor; MGC105282; OTTMUSP00000004170; OTTMUSP00000004171; RP23 - 394O9.2; TIMP 2; TIMP metallopeptidase inhibitor 2; Tissue inhibitor of metalloproteinase 2; Tissue inhibitor of metalloproteinases 2 |
| NCBI Gene ID: | 29543 |
| UniProt ID: | P30121 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 1.17 | 2.9 | 5.85 | 1.21 | 3.55 | 6.38 |
| Standard deviation: | 0.051 | 0.168 | 0.368 | 0.091 | 0.256 | 0.408 |
| CV (%): | 4.4 | 5.8 | 6.3 | 7.5 | 7.2 | 6.4 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.080 | 0.123 | 0.177 | 0.233 | 0.386 | 0.917 | 1.401 | 1.938 |
Images
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Rat TIMP-2 ELISA Kit standard curve |
Review