Overview
| Product Name: | Rat TIMP-1 ELISA Kit |
| Catalog No: | CUBEK01214 |
| Description: | For quantitative detection of rat TIMP - 1 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Rat |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant rat TIMP-1. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: C24 - A217 |
| Pinciple: | This rat IL-18 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forIL-18 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of rat IL-18 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 3 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
|
Material Required But Not Provided
|
Background
| Gene Name: | TIMP-1 |
| Background: | The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid - potentiating activity (EPA). The X - linked gene for human TIMP1 is expressed in some but not all inactive X - containing somatic - cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin - 3 (IL - 3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1 - p11.4.3 The standard product used in this kit is recombinant rat TIMP - 1, consisting of 194 amino acids with the molecular mass of 21.5KDa. As a result of glycosylation, the molecular mass is 32 - 34KDa. |
| Synonym: | C - C chemokine receptor type 5; C - C CKR - 5; CC - CKR - 5; CCR - 5; CCR5; CCR5_HUMAN; CD195; CHEMR13; CLGI; Collagenase inhibitor; EPA; EPO; Erythroid potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; HIV - 1 fusion coreceptor; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP; TIMP 1; TIMP metallopeptidase inhibitor 1; TIMP1 protein; Tissue Inhibitor of metalloproteinase 1; Tissue inhibitor of metalloproteinases; Tissue inhibitor of metalloproteinases 1; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor |
| NCBI Gene ID: | 116510 |
| UniProt ID: | P30120 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 162 | 524 | 1447 | 178 | 559 | 1308 |
| Standard deviation: | 7 | 32.5 | 73.8 | 13.17 | 36.34 | 90.25 |
| CV (%): | 4.3 | 6.2 | 5.1 | 7.4 | 6.5 | 6.9 |
Typical Data
| Concentration (pg/ml): | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D.: | 0.045 | 0.068 | 0.094 | 0.165 | 0.333 | 0.706 | 1.437 | 2.283 |
Images
 |
Rat TIMP-1 ELISA Kit standard curve |
Review