Overview
| Product Name: | Human AGRP/agouti-related ELISA Kit |
| Catalog No: | CUBEK01732 |
| Description: | For quantitative detection of human AGRP in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human AGRP. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: sf21; Immunogen sequence: A21 - T132 |
| Pinciple: | This mouse TPOR ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TPOR has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTPOR is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse TPOR within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 4 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | AGRP |
| Background: | Agouti - related protein, also called Agouti - related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY - containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti - related peptide is encoded by the AGRP gene. AgRP is co - expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long - lasting of appetite stimulators. |
| Synonym: | Agrp; AGRT; ART; ASIP2 |
| NCBI Gene ID: | 181 |
| UniProt ID: | O00253 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 42.7 | 155 | 347 | 50.8 | 192 | 420 |
| Standard deviation: | 1.84 | 7.60 | 19.43 | 3.05 | 10.75 | 26.88 |
| CV (%): | 4.3 | 4.9 | 5.6 | 6 | 5.6 | 6.4 |
Typical Data
| Concentration (pg/ml): | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D.: | 0.012 | 0.065 | 0.113 | 0.223 | 0.440 | 0.814 | 1.439 | 2.332 |
Review