Overview
Properties
Sensitivity: | < 10 pg/ml |
Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
Gene Name: | ADAM12 |
Background: | ADAM12 (Disintegrin and metalloproteinase domain - containing protein 12), also known as mlTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane - anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell - cell and cell - matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane - bound form. The shorter form is found to stimulate myogenesis. By RT - PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3. |
Synonym: | ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; ADAM12; Disintegrin and metalloproteinase domain - containing protein 12; MCMP; MCMP mltna; Meltrin - alpha; mlTN; mlTNA |
NCBI Gene ID: | 8038 |
UniProt ID: | O43184 |
Intra/Inter Assay Precision
Intra | Inter | |||||
Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
n: | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (ng/ml): | 1.5 | 4.2 | 7.4 | 1.6 | 4.1 | 7.2 |
Standard deviation: | 0.07 | 0.18 | 0.29 | 0.09 | 0.21 | 0.36 |
CV (%)\4.6; 4.3; 3.9; 5.6; 5.1; 5: | Notice: Undefined offset: 1 in /var/www/html_cubeab/catalog/view/theme/enar/template/product/product.tpl on line 475
Typical Data
Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D.: | 0.027 | 0.084 | 0.138 | 0.264 | 0.491 | 0.831 | 1.340 | 2.170 |
Images
Human ADAM12 ELISA Kit standard curve |
Review