Overview
| Product Name: | Human ACE2 ELISA Kit |
| Catalog No: | CUBEK01487 |
| Description: | For quantitative detection of human ACE2 in cell culture supernatants, serum and plasma (heparin). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ACE2. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: Q18 - S740 |
| Pinciple: | This rat TGF-beta 3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 3 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTGF-beta 3 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of rat TGF-beta 3 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | ACE2 |
| Background: | AngiotensinI - converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin - converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1 - 9, and angiotensin II into the vasodilator angiotensin 1 - 7. The organ - and cell - specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV - NL63. |
| Synonym: | ACE 2; ACE related carboxypeptidase; ACE - related carboxypeptidase; ACE2; ACE2_HUMAN; ACEH; Angiotensin converting enzyme 2; Angiotensin converting enzyme homolog; Angiotensin converting enzyme like protein; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2; Angiotensin I converting enzyme 2; Angiotensin - converting enzyme homolog; DKFZP434A014; EC 3.4.17; metalloprotease MPROT 15; Metalloprotease MPROT15; OTTHUMP00000022963; Processed angiotensin - converting enzyme 2 |
| NCBI Gene ID: | 59272 |
| UniProt ID: | Q9BYF1 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 438 | 1175 | 2386 | 508 | 1293 | 2417 |
| Standard deviation: | 19.71 | 56.4 | 124.07 | 34.54 | 98.27 | 188.53 |
| CV (%): | 4.5 | 4.8 | 5.2 | 6.8 | 7.6 | 7.8 |
Typical Data
| Concentration (pg/ml): | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D.: | 0.154 | 0.208 | 0.258 | 0.347 | 0.538 | 0.885 | 1.440 | 2.205 |
Images
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Human ACE2 ELISA Kit standard curve |
Review