Overview
Properties
Sensitivity: | < 5 pg/ml |
Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
Gene Name: | ACE |
Background: | Angiotensin - converting enzyme (ACE) is a zinc - containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C - terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732 - residue preprotein including a 31 - residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin - converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30 - 1261 amino acids with the molecular mass of 120KDa. |
Synonym: | ACE 1 ; ACE T ; Angiotensin converting enzyme ; Angiotensin Converting Enzyme 1 ; Angiotensinconverting enzyme ; Angiotensin - converting enzyme ; CD143 antigen ; DCP 1 ; DCP, DCP1 ; Dipeptidyl carboxypeptidase 1 ; Dipeptidyl carboxypeptidase I ; Kininase II ; MVCD3 ; P12821 ; Peptidyl dipeptidase A |
NCBI Gene ID: | 1636 |
UniProt ID: | B4DKH4 |
Intra/Inter Assay Precision
Intra | Inter | |||||
Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
n: | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (ng/ml): | 0.85 | 2.73 | 6.87 | 0.97 | 3.42 | 6.33 |
Standard deviation: | 0.036 | 0.112 | 0.261 | 0.671 | 0.246 | 0.335 |
CV (%): | 4.2 | 4.1 | 3.8 | 6.9 | 7.2 | 5.3 |
Typical Data
Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D.: | 0.051 | 0.119 | 0.204 | 0.336 | 0.611 | 0.929 | 1.447 | 2.154 |
Images
Human ACE ELISA Kit standard curve |
Review