Human A2M/alpha2-Macroglobulin ELISA Kit (CUBEK01502)

Product Name: Human A2M/alpha2-Macroglobulin ELISA Kit
Catalog No: CUBEK01502
Description: For quantitative detection of human A2M in cell culture supernatants, serum and plasma (heparin, EDTA).
Quantity: 12 X 8-well Strips (96T)
Target Species: Human
Tested Applications: ELISA
Specificity: Natural human A2M. There is no detectable cross-reactivity with other relevant proteins.
Standard Source: From human plasma
Pinciple: This human CCL16 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL16 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forCCL16 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human CCL16 within the sample that is captured by the plate.
Manual/Data Sheet: PDF:
Sensitivity: < 20 pg/ml
Storage Instructions: Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.)
Kit Components
Catalog NumberDescriptionQuantity
 96-well plate precoated with antibody1
CUBEK00501Lyophilized standard2 Tubes
Biotinylated anti-antibody130 µl (dilution 1:100)
 Avidin-Biotin-Peroxidase Complex (ABC)130 µl (dilution 1:100)
CUBEK00504Sample dilution buffer30 ml
CUBEK00505Antibody dilution buffer12 ml
CUBEK00506ABC dilution buffer12 ml
 TMB color developing agent10 ml
 TMB stop solution10 ml
 Adhesive cover4
Material Required But Not Provided
  1. Microplate reader in standard size.
  2. Automated plate washer.
  3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  4. Washing buffer (neutral PBS or TBS).
    •   - Preparation of 0.01 M TBS: Add 1.2 g Tris, 8.5 g NaCl; 450 µl of purified acetic acid or 700 µl of concentrated hydrochloric acid to 1000 ml H2O and adjust pH to 7.2 - 7.6. Finally, adjust the total volume to 1 L.
    •   - Preparation of 0.01 M PBS: Add 8.5 g sodium chloride, 1.4 g Na2HPO4 and 0.2 g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2 - 7.6. Finally, adjust the total volume to 1 L.
Gene Name: A2M
Background: Alpha - 2 - macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha - 2 - macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A - beta, the major component of beta - amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Synonym: C3 and PZP - like alpha - 2 - macroglobulin domain - containing protein 5; A2m; A2MD; A2MG_HUMAN; Alpha - 2 - M; Alpha - 2 - macroglobulin; C3 and PZP - like alpha - 2 - macroglobulin domain - containing protein 5; CPAMD5; FWP007; Pregnancy zone protein; S863 - 7
NCBI Gene ID: 2
UniProt ID: P01023
Intra/Inter Assay Precision
Intra Inter
Sample: 1 2 3 1 2 3
n: 16 16 16 24 24 24
Mean (ng/ml): 6.18 12.04 25.21 6.72 13.68 26.81
Standard deviation: 0.377 0.771 0.174 0.457 0.985 2.12
CV (%): 6.1 6.4 6.9 6.8 7.2 7.9
Typical Data
Concentration (pg/ml): 0 625 1250 2500 5000 10000 20000 40000
O.D.: 0.075 0.129 0.203 0.332 0.575 1.108 1.477 2.135

Human A2M/alpha2-Macroglobulin ELISA Kit standard curve


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Cat No.: CUBEK01502
Size Price
12 X 8-well Strips (96T) $369.00
Shipping info


Antibodies, Proteins, ELISA Kits: 5 to 10 Business Days, Ambient (unless otherwise required*)
*Based on stability testing and to save packing waste, most antibodies will be shipped at ambient temperature.

Cells: 2 to 3 Days, Dry Ice.


Ambient:  $25

Blue Ice/Ice Packs:  $45 (As Required or Upon request)

Dry Ice: $55



Order Info