Human 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit (CUBEK02192)

Product Name: Human 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit
Catalog No: CUBEK02192
Description: This ELISA kit applies to the in vitro quantitative determination of Human 5-LO concentrations in serum, plasma and other biological fluids.
Quantity: 12 X 8-well Strips (96T)
Target Species: Human
Tested Applications: ELISA
Specificity: This kit recognizes natural and recombinant human 5-LO. No significant cross-reactivity or interference between human 5-LO and analogues was observed.
Pinciple: The Human 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit uses the Sandwich-ELISA method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human 5-LO. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. A biotinylated detection antibody specific for human 5-LO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each well, successively, and the plate is incubated at 37˚C. The wells are washed and the substrate solution is added. Wells containing human 5-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme substrate is terminated by the addition of a sulfuric acid solution, turning the solution yellow. The optical density (OD) is measured spectrophotometrically at wavelength of 450 nm. The OD value is proportional to the concentration of the human 5-LO. You can calculate the concentration of human 5-LO in the samples by comparing the OD of the samples to the standard curve.
Manual/Data Sheet: PDF:
Sensitivity: 0.094 ng/ml
Assay Range: 0.156 - 10 ng/ml
Sample Type: Serum, Plasma, Biological Fluids
Storage Instructions: Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.)
Kit Components
Catalog NumberQuantityStorage
Micro ELISA Plate 8 wells ×12 strips4℃/-20℃
Reference Standard 2 tubes4℃/-20℃
Reference Standard & Sample Dilution Buffer20 ml4℃
Concentrated Biotinylated Detection Ab 120 µl4℃/-20℃
Biotinylated Detection Ab Dilution Buffer 10 ml4℃
Concentrated HRP Conjugate 120 µl4℃ (dark)
HRP Conjugate Diluent 10 ml4℃
Concentrated Wash Buffer (25×) 30 ml4℃
Substrate Reagent10 ml4℃ (dark)
Stop Solution10 ml4℃
Adhesive film5 
Material Required But Not Provided
  1. Microplate (450 nm) reader in standard size.
  2. Recommended: Automated plate washer.
  3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  4. Absorbent paper
Gene Name: 5-LO
Synonym: ALOX5, 5-LOX, LOX5, 5LPG, LOG5

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Cat No.: CUBEK02192
Size Price
12 X 8-well Strips (96T) $481.40
Shipping info


Antibodies, Proteins, ELISA Kits: 5 to 10 Business Days, Ambient (unless otherwise required*)
*Based on stability testing and to save packing waste, most antibodies will be shipped at ambient temperature.

Cells: 2 to 3 Days, Dry Ice.


Ambient:  $25

Blue Ice/Ice Packs:  $45 (As Required or Upon request)

Dry Ice: $55



Order Info