Product Name: GFP ELISA Kit
Catalog No: CUBEK01742

For quantitative detection of GFP in cell lysates and tissue homogenates.

Quantity: 12 X 8-well Strips (96T)
Target Species: GFP
Tested Applications: ELISA
Specificity: Natural and recombinant GFP. There is no detectable cross-reactivity with other relevant proteins.
Standard Source: Expression system for standard: E.coli; Immunogen sequence: S2 - K238
Pinciple: This mouse IDS ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IDS has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forIDS is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse IDS within the sample that is captured by the plate.
Manual/Data Sheet: PDF:
Sensitivity: < 10 pg/ml
Storage Instructions: Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.)
Kit Components
Catalog NumberDescriptionQuantity
 96-well plate precoated with antibody1
CUBEK00501Lyophilized standard2 Tubes
Biotinylated anti-antibody130 µl (dilution 1:100)
 Avidin-Biotin-Peroxidase Complex (ABC)130 µl (dilution 1:100)
CUBEK00504Sample dilution buffer30 ml
CUBEK00505Antibody dilution buffer12 ml
CUBEK00506ABC dilution buffer12 ml
 TMB color developing agent10 ml
 TMB stop solution10 ml
 Adhesive cover4
Material Required But Not Provided
  1. Microplate reader in standard size.
  2. Automated plate washer.
  3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  4. Washing buffer (neutral PBS or TBS).
    •   - Preparation of 0.01 M TBS: Add 1.2 g Tris, 8.5 g NaCl; 450 µl of purified acetic acid or 700 µl of concentrated hydrochloric acid to 1000 ml H2O and adjust pH to 7.2 - 7.6. Finally, adjust the total volume to 1 L.
    •   - Preparation of 0.01 M PBS: Add 8.5 g sodium chloride, 1.4 g Na2HPO4 and 0.2 g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2 - 7.6. Finally, adjust the total volume to 1 L.
Gene Name: GFP
Background: The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof - of - concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human.
Synonym: GFP; Green fluorescent protein; yfp
UniProt ID: P42212
Intra/Inter Assay Precision
Intra Inter
Sample: 1 2 3 1 2 3
n: 16 16 16 24 24 24
Mean (pg/ml): 96 351 668 115 427 722
Standard deviation: 4.61 24.22 41.42 6.44 30.32 53.43
CV (%): 4.8 6.9 6.2 5.6 7.1 7.4
Typical Data
Concentration (pg/ml): 0 15.6 31.2 62.5 125 250 500 1000
O.D.: 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225

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Cat No.: CUBEK01742
Size Price
12 X 8-well Strips (96T) $333.00
Shipping info


Antibodies, Proteins, ELISA Kits: 5 to 10 Business Days, Ambient (unless otherwise required*)
*Based on stability testing and to save packing waste, most antibodies will be shipped at ambient temperature.

Cells: 2 to 3 Days, Dry Ice.


Ambient:  $25

Blue Ice/Ice Packs:  $45 (As Required or Upon request)

Dry Ice: $55



Order Info