Human 12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit (CUBEK02184)

Product Name: Human 12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit
Catalog No: CUBEK02184
Description: This ELISA kit applies to the in vitro quantitative determination of Human 12-HETE concentrations in serum, plasma and other biological fluids.
Quantity: 12 X 8-well Strips (96T)
Target Species: Human
Tested Applications: ELISA
Specificity: This kit recognizes natural and recombinant human 12-HETE. No significant cross-reactivity or interference between human 12-HETE and analogues was observed.
Pinciple: The Human 12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit uses the Sandwich-ELISA method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human 12-HETE. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. A biotinylated detection antibody specific for human 12-HETE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each well, successively, and the plate is incubated at 37˚C. The wells are washed and the substrate solution is added. Wells containing human 12-HETE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme substrate is terminated by the addition of a sulfuric acid solution, turning the solution yellow. The optical density (OD) is measured spectrophotometrically at wavelength of 450 nm. The OD value is proportional to the concentration of the human 12-HETE. You can calculate the concentration of human 12-HETE in the samples by comparing the OD of the samples to the standard curve.
Manual/Data Sheet: PDF:
Sensitivity: 0.469 ng/ml
Assay Range: 0.781 - 50 ng/ml
Sample Type: Serum, Plasma, Biological Fluids
Storage Instructions: Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.)
Kit Components
Catalog NumberQuantityStorage
Micro ELISA Plate 8 wells ×12 strips4℃/-20℃
Reference Standard 2 tubes4℃/-20℃
Reference Standard & Sample Dilution Buffer20 ml4℃
Concentrated Biotinylated Detection Ab 120 µl4℃/-20℃
Biotinylated Detection Ab Dilution Buffer 10 ml4℃
Concentrated HRP Conjugate 120 µl4℃ (dark)
HRP Conjugate Diluent 10 ml4℃
Concentrated Wash Buffer (25×) 30 ml4℃
Substrate Reagent10 ml4℃ (dark)
Stop Solution10 ml4℃
Adhesive film5 
Material Required But Not Provided
  1. Microplate (450 nm) reader in standard size.
  2. Recommended: Automated plate washer.
  3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  4. Absorbent paper
Gene Name: 12-HETE
Synonym: 12-HETE

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Cat No.: CUBEK02184
Size Price
12 X 8-well Strips (96T) $481.40
Shipping info


Antibodies, Proteins, ELISA Kits: 5 to 10 Business Days, Ambient (unless otherwise required*)
*Based on stability testing and to save packing waste, most antibodies will be shipped at ambient temperature.

Cells: 2 to 3 Days, Dry Ice.


Ambient:  $25

Blue Ice/Ice Packs:  $45 (As Required or Upon request)

Dry Ice: $55



Order Info