Human P53 ELISA Kit (CUBEK01400)

Product Name: Human P53 ELISA Kit
Catalog No: CUBEK01400
Description: For quantitative detection of human P53 in cell lysates.
Quantity: 12 X 8-well Strips (96T)
Target Species: Human
Tested Applications: ELISA
Specificity: Natural and recombinant human P53. There is no detectable cross-reactivity with other relevant proteins.
Standard Source: Expression system for standard: NSO; Immunogen sequence: M1 - D393
Pinciple: This rat MCP-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MCP-1 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forMCP-1 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of rat MCP-1 within the sample that is captured by the plate.
Manual/Data Sheet: PDF:
Sensitivity: < 10 pg/ml
Storage Instructions: Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.)
Kit Components
Catalog NumberDescriptionQuantity
 96-well plate precoated with antibody1
CUBEK00501Lyophilized standard2 Tubes
Biotinylated anti-antibody130 µl (dilution 1:100)
 Avidin-Biotin-Peroxidase Complex (ABC)130 µl (dilution 1:100)
CUBEK00504Sample dilution buffer30 ml
CUBEK00505Antibody dilution buffer12 ml
CUBEK00506ABC dilution buffer12 ml
 TMB color developing agent10 ml
 TMB stop solution10 ml
 Adhesive cover4
Material Required But Not Provided
  1. Microplate reader in standard size.
  2. Automated plate washer.
  3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  4. Washing buffer (neutral PBS or TBS).
    •   - Preparation of 0.01 M TBS: Add 1.2 g Tris, 8.5 g NaCl; 450 µl of purified acetic acid or 700 µl of concentrated hydrochloric acid to 1000 ml H2O and adjust pH to 7.2 - 7.6. Finally, adjust the total volume to 1 L.
    •   - Preparation of 0.01 M PBS: Add 8.5 g sodium chloride, 1.4 g Na2HPO4 and 0.2 g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2 - 7.6. Finally, adjust the total volume to 1 L.
Gene Name: P53
Background: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53 - kilodalton (kDa) protein on SDS - PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full - length p53. It has played a vital role in conserving stability by preventing genome mutation.
Synonym: Antigen NY - CO - 13; BCC7; Cellular tumor antigen p53; LFS1; MS980; p53; p53 tumor suppressor; P53_HUMAN; Phosphoprotein p53; Tp53; Transformation related protein 53; TRP53; Tumor suppressor p53
NCBI Gene ID: 7157
UniProt ID: K7PPA8
Intra/Inter Assay Precision
Intra Inter
Sample: 1 2 3 1 2 3
n: 16 16 16 24 24 24
Mean (pg/ml): 1328 2789 5434 1502 2891 6120
Standard deviation: 78.4 173 353.21 103.7 214 508
CV (%): 5.9 6.2 6.5 6.9 7.4 8.3
Typical Data
Concentration (pg/ml): 0 156 312 625 1250 2500 5000 10000
O.D.: 0.028 0.071 0.120 0.225 0.365 0.664 1.283 2.001

Human P53 ELISA Kit standard curve


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Cat No.: CUBEK01400
Size Price
12 X 8-well Strips (96T) $420.00
Shipping info


Antibodies, Proteins, ELISA Kits: 5 to 10 Business Days, Ambient (unless otherwise required*)
*Based on stability testing and to save packing waste, most antibodies will be shipped at ambient temperature.

Cells: 2 to 3 Days, Dry Ice.


Ambient:  $25

Blue Ice/Ice Packs:  $45 (As Required or Upon request)

Dry Ice: $55



Order Info