Product Name: | ViroVision™ Rev-CEM-Luc HIV Reporter Cells |
Catalog No: | CUBRC0033 |
Description: | Infection of Rev-CEM-Luc by HIV results in GFP expression in 2-5 days. Rev-CEM-Luc is permissive to a multi-subtype range of X4 utilizing primary isolates and infectious molecular clones. Rev-CEM-Luc has no background GFP expression in the absence of HIV infection and is resistant to non-viral stimulations by mitogens, cytokines, or other cellular activators. |
Quantity: | One vial of 5 x 10⁶ cells. |
Progenitor: | Human T4-lymphoblastoid cell line, CEM-SS. |
Phenotype: | Expresses CD4 and CXCR4. |
Applications: | TCID50 assays, routine measurements of viral infectivity, anti-HIV drug screening, anti-HIV neutralizing antibody screening and quantification, HIV cell-cell transmission, HIV+ cell-derived exosomes. |
Freezing Media: | ViroVision™ Freezing Media: 93% FBS/RPMI with 7% DMSO. |
Propagation Media: | ViroVision™ Growth Media C: RPMI, 10% FBS. |
Growth: | Split 1:2 every 1-2 days, or 1:10-1:20 every 2-3 days. |
Morphology: | Round, Clusters |
Detection: | Luminometer and luciferase detection system (Luc). |
Shipping Conditions: | Cells are shipped frozen. If cells are not frozen upon arrival, contact Cube Biosystems immediately. |
Storage Conditions: | Store cells in liquid nitrogen until ready to propagate. Store Infection Enhancment Media at -20°C until ready to use. Once thawed, Infection media is good for 3 months and should be stored at 4°C. |
Warranty: | Cube Biosystems warrants that cells shall be viable upon shipment from Cube Biosystems for a period of thirty days, provided they have been properly stored and handled during this period. |
Safety: | BSL 1: Appropriate safety procedures should always be used with this material. Prior to thawing cells, under sterile conditions: Unscrew cap 1/4 to 1/2 turn to allow N2 to escape. Re-secure cap. |
Quality: | Negative for mycoplasma, bacteria and fungi. |
Disclaimer: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. |
License: | PDF: Web: Click Here |
Directions for Use: | PDF: Web: Click Here |
Data Sheet: | PDF: |
Storage Instructions: | Store cells in vapor phase of liquid nitrogen until you are ready to thaw and propagate. |