Overview
| Product Name: | GFP ELISA Kit |
| Catalog No: | CUBEK01742 |
| Description: | For quantitative detection of GFP in cell lysates and tissue homogenates. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | GFP |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant GFP. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: E.coli; Immunogen sequence: S2 - K238 |
| Pinciple: | This mouse IDS ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IDS has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forIDS is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse IDS within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | GFP |
| Background: | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof - of - concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. |
| Synonym: | GFP; Green fluorescent protein; yfp |
| UniProt ID: | P42212 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 96 | 351 | 668 | 115 | 427 | 722 |
| Standard deviation: | 4.61 | 24.22 | 41.42 | 6.44 | 30.32 | 53.43 |
| CV (%): | 4.8 | 6.9 | 6.2 | 5.6 | 7.1 | 7.4 |
Typical Data
| Concentration (pg/ml): | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D.: | 0.055 | 0.137 | 0.182 | 0.295 | 0.496 | 0.897 | 1.563 | 2.225 |
Review