Overview
| Product Name: | Human AGP1/alpha 1 acid glycoprotein ELISA Kit |
| Catalog No: | CUBEK01766 |
| Description: | For quantitative detection of human AGP1 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human AGP1. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: Q19 - S201 |
| Pinciple: | This human LRIG3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LRIG3 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forLRIG3 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human LRIG3 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 150 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | AGP1 |
| Background: | Alpha - 1 - acid glycoprotein, or orosomucoid, is a relatively abundant plasma protein synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180 amino acids. AGP is considered an acute phase reactant; its plasma levels increase several fold during acute infections. It belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. And this gene is located in 9q34.3 - qter. |
| Synonym: | AGP 1 ; AGP1 ; AGP - 1 ; AGP A ; AGPA ; AGP ; OMD 1 ; ORM ; ORM1 ; Orosomucoid 1 ; Orosomucoid - 1 ; P02763 |
| NCBI Gene ID: | 5004 |
| UniProt ID: | P02763 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 7.3 | 28.5 | 63.7 | 8.4 | 32.8 | 78.9 |
| Standard deviation: | 0.489 | 1.795 | 4.905 | 0.663 | 2.788 | 7.022 |
| CV (%): | 6.7 | 6.3 | 7.7 | 7.9 | 8.5 | 8.9 |
Typical Data
| Concentration (ng/ml): | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D.: | 0.024 | 0.147 | 0.248 | 0.431 | 0.857 | 1.390 | 1.932 | 2.243 |
Review