Overview
| Product Name: | Human AFP ELISA Kit |
| Catalog No: | CUBEK01230 |
| Description: | For quantitative detection of human AFP in cell culture supernatants, serum and plasma ( heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human AFP. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: R19 - V609 |
| Pinciple: | This human TNFRSF17 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF17 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTNFRSF17 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human TNFRSF17 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | AFP |
| Background: | Alpha - fetoprotein (AFP, α - fetoprotein; also sometimes called alpha - 1 - fetoprotein, alpha - fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear. |
| Synonym: | Afp; Alpha fetoglobulin; Alpha - fetoglobulin; Alpha fetoprotein; Alpha - fetoprotein; Alpha - 1 - fetoprotein; FETA; HPAFP |
| NCBI Gene ID: | 174 |
| UniProt ID: | P02771 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 784 | 1507 | 3564 | 817 | 1785 | 4516 |
| Standard deviation: | 49.39 | 97.96 | 185.33 | 65.36 | 130.31 | 365.80 |
| CV (%): | 6.3 | 6.5 | 5.2 | 8 | 7.3 | 8.1 |
Typical Data
| Concentration (pg/ml): | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D.: | 0.003 | 0.137 | 0.248 | 0.486 | 0.777 | 1.260 | 1.704 | 1.925 |
Review