Overview
| Product Name: | Human ADAM15 ELISA Kit |
| Catalog No: | CUBEK01765 |
| Description: | For quantitative detection of human ADAM15 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ADAM15. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: D207 - T696 |
| Pinciple: | This human TAFI ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TAFI has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTAFI is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human TAFI within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 12 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | ADAM15 |
| Background: | Disintegrin and metalloproteinase domain - containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc - binding metalloprotease domain, a disintegrin - like domain, as well as an EGF - like domain. Through its disintegrin - like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein - tyrosine kinases in a phosphorylation - dependent manner, suggesting that this protein may function in cell - cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Synonym: | ADAM15 ; ADAM 15 ; MDC15 ; MDC 15 ; MDC - 15 ; Metargidin ; Q13444 |
| NCBI Gene ID: | 8751 |
| UniProt ID: | Q13444 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 366 | 1287 | 2453 | 412 | 1339 | 2874 |
| Standard deviation: | 27.81 | 113.25 | 181.52 | 35.43 | 123.19 | 267.28 |
| CV (%): | 7.6 | 8.8 | 7.4 | 8.6 | 9.2 | 9.3 |
Typical Data
| Concentration (pg/ml): | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D.: | 0.061 | 0.083 | 0.109 | 0.185 | 0.404 | 0.664 | 1.373 | 2.079 |
Review