Overview
| Product Name: | Human ADA ELISA Kit |
| Catalog No: | CUBEK01746 |
| Description: | For quantitative detection of human ADA in cell culture supernatants, serum, tissue homogenates and pleural fluid. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human ADA. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: sf21; Immunogen sequence: A2 - L363 |
| Pinciple: | This mouse FABP4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FABP4 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forFABP4 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of mouse FABP4 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | ADA |
| Background: | Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF - 1 alpha, which in part explains the attenuated levels of adenosine in HIF - 1 alpha expressing hearts during ischemic stress. |
| Synonym: | ada; ADA1; ADA 1; Adenosine aminohydrolase; Adenosine deaminase |
| NCBI Gene ID: | 100 |
| UniProt ID: | P00813 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 45 | 153 | 679 | 51 | 201 | 821 |
| Standard deviation: | 2.47 | 7.49 | 34.62 | 1.98 | 8.44 | 37.76 |
| CV (%): | 5.5 | 4.9 | 5.1 | 3.9 | 4.2 | 4.6 |
Typical Data
| Concentration (pg/ml): | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D.: | 0.051 | 0.118 | 0.196 | 0.299 | 0.574 | 0.995 | 1.753 | 2.449 |
Review