Overview
| Product Name: | Human Activin A ELISA Kit |
| Catalog No: | CUBEK01001 |
| Description: | For quantitative detection of human Activin A in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human Activin A. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: CHO; Immunogen sequence: G311 - S426 |
| Pinciple: | This human Activin A ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection monoclonal antibody from mouse specific for Activin A is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human Activin A within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 12 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | Activin A |
| Background: | Activin A is a homodimer of 14kDa beta - A. Activin A, a cytokine member of the transforming growth factor - beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa. |
| Synonym: | Activin beta A chain; erythroid differentiation factor; erythroid differentiation protein; follicle stimulating hormone releasing protein; FSH releasing protein; Inhibin; inhibin beta A chain |
| NCBI Gene ID: | 3624 |
| UniProt ID: | A4D1W7 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 90 | 281 | 467 | 95 | 293 | 489 |
| Standard deviation: | 4.7 | 15.7 | 27.6 | 7.8 | 18.5 | 24.9 |
| CV (%): | 5.2 | 5.6 | 5.9 | 8.2 | 6.3 | 5.1 |
Typical Data
| Concentration (pg/ml): | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D.: | 0.037 | 0.073 | 0.085 | 0.147 | 0.289 | 0.537 | 1.066 | 2.200 |
Review