Mouse TP53 (Tumor Protein 53) ELISA Kit (CUBEK05504)

Overview
Product Name: Mouse TP53 (Tumor Protein 53) ELISA Kit
Catalog No: CUBEK05504
Description: This ELISA kit applies to the in vitro quantitative determination of Mouse TP53 concentrations in serum, plasma and other biological fluids.
Quantity: 12 X 8-well Strips (96T)
Target Species: Mouse
Tested Applications: ELISA
Specificity: This kit recognizes natural and recombinant mouse TP53. No significant cross-reactivity or interference between mouse TP53 and analogues was observed.
Pinciple: The Mouse TP53 (Tumor Protein 53) ELISA Kit uses the Sandwich-ELISA method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to mouse TP53. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. A biotinylated detection antibody specific for mouse TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each well, successively, and the plate is incubated at 37˚C. The wells are washed and the substrate solution is added. Wells containing mouse TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme substrate is terminated by the addition of a sulfuric acid solution, turning the solution yellow. The optical density (OD) is measured spectrophotometrically at wavelength of 450 nm. The OD value is proportional to the concentration of the mouse TP53. You can calculate the concentration of mouse TP53 in the samples by comparing the OD of the samples to the standard curve.
Manual/Data Sheet: PDF:
SDS: SDS:
Properties
Sensitivity: 37.5 pg/ml
Assay Range: 62.5 - 4000 pg/ml
Sample Type: Serum, Plasma, Biological Fluids
Storage Instructions: Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.)
Kit Components
Catalog NumberQuantityStorage
Micro ELISA Plate 8 wells ×12 strips4℃/-20℃
Reference Standard 2 tubes4℃/-20℃
Reference Standard & Sample Dilution Buffer20 ml4℃
Concentrated Biotinylated Detection Ab 120 µl4℃/-20℃
Biotinylated Detection Ab Dilution Buffer 10 ml4℃
Concentrated HRP Conjugate 120 µl4℃ (dark)
HRP Conjugate Diluent 10 ml4℃
Concentrated Wash Buffer (25×) 30 ml4℃
Substrate Reagent10 ml4℃ (dark)
Stop Solution10 ml4℃
Adhesive film5 
Material Required But Not Provided
  1. Microplate (450 nm) reader in standard size.
  2. Recommended: Automated plate washer.
  3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  4. Absorbent paper
Background
Gene Name: TP53
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Cat No.: CUBEK05504
Size Price
12 X 8-well Strips (96T) $481.40
Shipping info

Delivery

Antibodies, Proteins, ELISA Kits: 5 to 10 Business Days, Ambient (unless otherwise required*)
*Based on stability testing and to save packing waste, most antibodies will be shipped at ambient temperature.

Cells: 2 to 3 Days, Dry Ice.

Charges

Ambient:  $25

Blue Ice/Ice Packs:  $45 (As Required or Upon request)

Dry Ice: $55

                            

 

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