Overview
| Product Name: | Human sCD40L ELISA Kit |
| Catalog No: | CUBEK01205 |
| Description: | For quantitative detection of human soluble CD40L in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human CD40L. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: E.coli; Immunogen sequence: E108 - L261 |
| Pinciple: | This human sCD40LG ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sCD40LG has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific for sCD40LG is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human GH within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 15 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | CD40L |
| Background: | CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor - alpha and - beta, and has important functions in B - cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3 - 27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions. |
| Synonym: | CD154; CD40 antigen ligand; CD40 ligand; CD40 - L; CD40L_HUMAN; CD40LG; gp39; HIGM1; IGM; IMD3; soluble form; T B cell activating molecule; T BAM; T cell antigen Gp39; T - cell antigen Gp39; TNF related activation protein; TNF - related activation protein; TNFSF5; TrAP; Tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor (ligand) superfamily, member 5 (hyper - IgM syndrome); Tumor necrosis factor ligand superfamily member 5 |
| NCBI Gene ID: | 959 |
| UniProt ID: | P29965 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 0.44 | 1.26 | 2.38 | 0.42 | 1.41 | 2.46 |
| Standard deviation: | 0.026 | 0.064 | 0.145 | 0.029 | 0.086 | 0.182 |
| CV (%): | 5.9 | 5.1 | 6.1 | 6.8 | 6.1 | 7.4 |
Typical Data
| Concentration (pg/ml): | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D.: | 0.084 | 0.148 | 0.194 | 0.337 | 0.518 | 1.035 | 1.572 | 2.111 |
Images
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Review