Overview
| Product Name: | Human PECAM-1/CD31 ELISA Kit |
| Catalog No: | CUBEK01391 |
| Description: | For quantitative detection of human PECAM - 1 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human PECAM-1. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: CHO; Immunogen sequence: Q28 - K601 |
| Pinciple: | This human BMP-7 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-7 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forBMP-7 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human BMP-7 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | PECAM-1 |
| Background: | Human platelet/endothelial cell adhesion molecule - 1 (PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23 - qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM - 1 in regulating platelet - collagen interactions, 2 different approaches were applied using recombinant human PECAM - 1 - immunoglobulin chimeras and platelets derived from PECAM - 1 - deficient mice.3 PECAM - 1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 574 amino acids (Q28 - K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90 - 100KDa. |
| Synonym: | CD31; CD31 antigen; EndoCAM; GPIIA; HEC7; Leukocyte surface protein; PECA1; PECA1_HUMAN; PECAM 1; PECAM - 1; Pecam1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule |
| NCBI Gene ID: | 5175 |
| UniProt ID: | P16284 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 1282 | 2520 | 5023 | 1526 | 2768 | 5281 |
| Standard deviation: | 87.2 | 120.96 | 261.2 | 116 | 182.7 | 338 |
| CV (%): | 6.8 | 4.8 | 5.2 | 7.6 | 6.6 | 6.4 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.086 | 0.176 | 0.284 | 0.400 | 0.696 | 1.145 | 1.629 | 2.207 |
Images
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Human PECAM-1/CD31 ELISA Kit standard curve |
Review