Overview
| Product Name: | Human P53 ELISA Kit |
| Catalog No: | CUBEK01400 |
| Description: | For quantitative detection of human P53 in cell lysates. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human P53. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: M1 - D393 |
| Pinciple: | This rat MCP-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MCP-1 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forMCP-1 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of rat MCP-1 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | P53 |
| Background: | P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53 - kilodalton (kDa) protein on SDS - PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full - length p53. It has played a vital role in conserving stability by preventing genome mutation. |
| Synonym: | Antigen NY - CO - 13; BCC7; Cellular tumor antigen p53; LFS1; MS980; p53; p53 tumor suppressor; P53_HUMAN; Phosphoprotein p53; Tp53; Transformation related protein 53; TRP53; Tumor suppressor p53 |
| NCBI Gene ID: | 7157 |
| UniProt ID: | K7PPA8 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 1328 | 2789 | 5434 | 1502 | 2891 | 6120 |
| Standard deviation: | 78.4 | 173 | 353.21 | 103.7 | 214 | 508 |
| CV (%): | 5.9 | 6.2 | 6.5 | 6.9 | 7.4 | 8.3 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.028 | 0.071 | 0.120 | 0.225 | 0.365 | 0.664 | 1.283 | 2.001 |
Images
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Human P53 ELISA Kit standard curve |
Review