Overview
| Product Name: | Human Kallistatin/Serpina4 ELISA Kit |
| Catalog No: | CUBEK01365 |
| Description: | For quantitative detection of human Kallistatin in cell culture supernatants, serum and plasma (heparin, EDTA). |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human Kallistatin. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: Q21 - P427 |
| Pinciple: | This human TrkA ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTrkA is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human TrkA within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | Kallistatin |
| Background: | Serpin peptidase inhibitor, clade A (alpha - 1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin - binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth. |
| Synonym: | KAIN_HUMAN; Kallikrein inhibitor; Kallistatin; Peptidase inhibitor 4; PI - 4; PI4; Serpin A4; SERPINA4 |
| NCBI Gene ID: | 5267 |
| UniProt ID: | P29622 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml): | 1521 | 3786 | 6866 | 1637 | 4242 | 7128 |
| Standard deviation: | 77.6 | 170.4 | 425.7 | 104.8 | 237.6 | 499 |
| CV (%): | 5.1 | 4.5 | 6.2 | 6.4 | 5.6 | 7 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.067 | 0.125 | 0.175 | 0.280 | 0.450 | 0.848 | 1.489 | 2.153 |
Images
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Human Kallistatin/Serpina4 ELISA Kit standard curve |
Review