Overview
| Product Name: | Human Endostatin ELISA Kit |
| Catalog No: | CUBEK01398 |
| Description: | For quantitative detection of human Endostatin in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human Endostatin. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: E.coli; Immunogen sequence: H1154 - S1335 |
| Pinciple: | This human THBS1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for THBS1 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forTHBS1 is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human THBS1 within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | Endostatin |
| Background: | Endostatin is a naturally - occurring 20 - kDa C - terminal fragment derived from type XVIII collagen. It is reported to serve as an anti - angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo. |
| Synonym: | Alpha 1 collagen type 18 (XVIII) (COL18A1); Alpha 1 type XVIII collagen; Antiangiogenic agent; COL18A1; Collagen alpha 1 (XVIII) chain; Collagen type XVIII alpha 1; FLJ27325; FLJ34914; KNO; KNO1; MGC74745; Multi functional protein MFP; OTTMUSP00000023764; OTTMUSP00000023766; Procollagen type XVIII alpha 1 |
| NCBI Gene ID: | 80781 |
| UniProt ID: | P39060 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 0.75 | 2.59 | 5.21 | 0.89 | 2.46 | 5.16 |
| Standard deviation: | 0.054 | 0.168 | 0.224 | 0.072 | 0.177 | 0.346 |
| CV (%): | 7.2 | 6.5 | 4.3 | 8.1 | 7.2 | 6.7 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.068 | 0.294 | 0.596 | 0.980 | 1.323 | 1.555 | 1.695 | 1.916 |
Images
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Human Endostatin ELISA Kit standard curve |
Review