Overview
Properties
Sensitivity: | < 10 pg/ml |
Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
|
Material Required But Not Provided
|
Background
Gene Name: | Cystatin C |
Background: | Cystatin C or cystatin 3 (formerly gamma trace, post - gamma - globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4 |
Synonym: | ARMD11; bA218C14.4 (cystatin C); Cst3; Cystatin - 3; Cystatin - C; CYTC_HUMAN; Gamma - trace; Neuroendocrine basic polypeptide; Post - gamma - globulin |
NCBI Gene ID: | 1471 |
UniProt ID: | P01034 |
Intra/Inter Assay Precision
Intra | Inter | |||||
Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
n: | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (ng/ml): | 2.68 | 6.13 | 12.83 | 2.87 | 6.24 | 13.21 |
Standard deviation: | 0.115 | 0.288 | 0.744 | 0.152 | 0.356 | 0.845 |
CV (%): | 4.3 | 4.7 | 5.8 | 5.3 | 5.7 | 6.4 |
Typical Data
Concentration (pg/ml): | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D.: | 0.062 | 0.143 | 0.212 | 0.347 | 0.582 | 0.992 | 1.444 | 1.836 |
Images
Human Cystatin C ELISA Kit standard curve |
Review