Overview
| Product Name: | Human Angiostatin K1-3 ELISA Kit |
| Catalog No: | CUBEK01409 |
| Description: | For quantitative detection of human Angiostatin K1 - 3 in serum, plasma and cell culture supernatants. |
| Quantity: | 12 X 8-well Strips (96T) |
| Target Species: | Human |
| Tested Applications: | ELISA |
| Specificity: | Natural and recombinant human Angiostatin K1-3. There is no detectable cross-reactivity with other relevant proteins. |
| Standard Source: | Expression system for standard: NSO; Immunogen sequence: V98 - S355 (K1 - 3) |
| Pinciple: | This human Clusterin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Clusterin has been pre-coated onto 96-well plates. Standards and test samples are added to the wells. A biotinylated detection polyclonal antibody from goat specific forClusterin is added, followed by washing with PBS or TBS buffer. An Avidin-Biotin-Peroxidase Complex is added and the unbound conjugates are washed away with PBS or TBS buffer. HRP substrate (TMB) is used to visualize the HRP enzymatic reaction. The HRP-TMB reaction initially produces a blue color that changes yellow upon adding the acidic stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the amount of human Clusterin within the sample that is captured by the plate. |
| Manual/Data Sheet: | PDF:  |
| SDS: | SDS: |
Properties
| Sensitivity: | < 10 pg/ml |
| Storage Instructions: | Store at 4˚C for 1 month, at -20˚C for up to longer storage. Avoid multiple freeze/thaw cycles (Shipped with blue ice.) |
Kit Components
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Material Required But Not Provided
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Background
| Gene Name: | Angiostatin K1-3 |
| Background: | Ang K1 - 3 is a single, non - glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1 - 3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED₅₀ =70 nM) relative to kringles 1 - 4 (ED₅₀ = 135 nM). |
| Synonym: | Ang K1 - 3 |
| NCBI Gene ID: | 5340 |
| UniProt ID: | P00747 |
Intra/Inter Assay Precision
| Intra | Inter | |||||
| Sample: | 1 | 2 | 3 | 1 | 2 | 3 |
| n: | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (ng/ml): | 1.09 | 3.68 | 6.96 | 1.13 | 3.75 | 7.04 |
| Standard deviation: | 0.041 | 0.155 | 0.320 | 0.057 | 0.199 | 0.415 |
| CV (%): | 3.8 | 4.2 | 4.6 | 5 | 5.3 | 5.9 |
Typical Data
| Concentration (pg/ml): | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D.: | 0.015 | 0.132 | 0.253 | 0.423 | 0.746 | 1.355 | 1.827 | 2.310 |
Images
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Human Angiostatin K1-3 ELISA Kit standard curve |
Review